摘要
在桐叶槭(Acer pseudoplatanus L.)悬浮培养细胞的膜组分上有 NPA(N-1-naphthylpathalamic acid)专一结合位点。它与NPA有高亲和力。它们的平衡解离常数(Kd值)为 7.5×10^(-9)mol/L。在4℃保温 60分钟,NPA的结合量达最高点,平衡状态至少可维持2小时。人工合成的生长素类物质:1-NAA(1-萘乙酸)、2-NAA、2,4-D以及生长素运输抑制剂2,3,5-TIBA(三碘苯甲酸)均能与NPA竞争同一结合位点。这些物质与此结合位点的Kd值范围为 10^(-4)—10^(-6)mol/L之间。实验表明:NPA结合位点可能在调节细胞内IAA水平上起作用,从而影响细胞的生长。
A specific NPA N-1-naphthylpathalamic acid)-binding site with high affinity was found in the membrane component of Acer pseudoplatanus L. suspension culture cells. The dissocia- tion constant (Kd) of NPA was approximately 7.5×10^(-9)mol/L. NPA binding was maximal after 60 min of incubation at 4℃. Synthetic auxin 1-NAA, 2-NAA, 2,4-D and auxin trans- port inhibitor 2,3,5-TIBA were all able to compete with NPA for the binding. Site with Kd values ranging from 10^(-6) to 10^(-4) mol/L. These binding sites could be involved in the regula- tion of intracellular IAA level, thereby affecting cellgrowth.
基金
国家自然科学基金
