摘要
将编码番茄核酮糖－１，５－二磷酸羧化酶／加氧酶小亚基转运肽的一段ＤＮＡ序列与菠菜Ｒｕｂｉｓｃｏ大亚基的编码区连接，构建了一个Ｒｕｂｉｓｃｏ融合基因。限制性内切酶图谱和ＤＮＡ序列分析证明副合部位的核苷酸序列符合构建前的三联密码子框架。将Ｒｕｂｉｓｃｏ融合基因转入Ｅ．ｃｏｌｉ，用ＩＰＴＧ进行诱导表达。利用蛋白质印迹技术检测到诱导产物的存在。
A fusion gene was constructed with the signal sequence of Rubisco (ribulose-1,5- bisphosphate carboxylase/oxygenase) small subunit from tomato and the coding region of Rubisco large subunit from spinach. The fusion gene was confirmed with restriction endonucleases and DNA sequencing analysis for the open reading frame. The chimeric gene was transferred to E. coli and its expression was induced by addition of IPTG. Expression of the Rubisco fusion gene was detected by Western blotting.
基金
国家自然科学基金
国家教委博士点专项基金资助项目
关键词
二磷酸核酮糖
羧化酶
氧酶
基因
Ribulose-1
5-bisphosphate carboxylase/oxygenase
Transit peptide
Fusion gene
