叶绿体腺三磷酶中色氨酸残基的修饰与性质的研究

STUDIES ON TRYPTOPHANE RESIDUES IN CF1-ATPase AND THE ENZYMATIC PROPERTY OF CF1-ATPase MODIFIED BY NBS AND PHOTOOXIDATION

利用Ｎ－溴代琥珀酰亚胺化学修饰叶绿体腺三磷酶，测得该该酶含３．５个色氨酸残基，其中２个色氨酸残基与酶活性有关。光氧化腺三磷酶伴随吸氧量增加，酶活性逐渐降低，经ＮＢＳ修饰或光氧化的腺三磷酶，其免疫抗原减弱。表明腺三磷酶中色氨酸基参与酶的活性和抗原性。

The numbers of tryptophane residues in spinach CF_1-ATPase were measured by means of chemical modification with N-bromosuccinimide (NBS) and photooxidation. There are 3. 5 tryptophane residues in CF_1-ATPase, among which two are essential for the enzyme activity. Photooxidation of CF_1-ATPase led to increased O_2 uptake of the reaction system and loss in activity of CF_1-ATPase . Immunological property of CF_1-ATPase has been altered by chemical modification with NBS and photooxidation. The results show that tryptophane residues seen to be essential for activity and antigenic properties of CF_1-ATPase .