冬虫夏草发酵菌丝体中主要核苷及核酸碱基的HPLC测定

HPLC Determination of Main Nucleoside and Nucleic Acid Base in Mycelia of Cordyceps

采用HPLC法测定四种人工培养冬虫夏草发酵菌丝体产品中六个核苷及碱基成分。着重对其中的腺苷成分作了提取溶剂比较及回收率试验等。采用水为提取溶剂,提取效率较50%乙醇为高;水中加入0.1％叠氮钠,既能防腐,又对样液中核苷成分起稳定作用。超声波室温下提取,加样回收率为99.8%。

Six nucleosides and base components in Four products of Mycelia of cultivated Cordyceps were determined. The comparison of extraction solvents and recovery test of the adenosine components were emphasized. The effective extraction-rate by water,as an extraction solvent, was higher than that by 50% alcohol. The addition of 0. 1% sodium azide into water could not only preserve putrefaction,but also make a contribution to the stability of nucleoside components in the liquid sample. When the extraction was carried out by ultrasonic at room temperature,the recovery rate of the added sample was 99. 8%.