脑缺血再灌注过程中大鼠脑皮质LPO、SOD和Ach含量的变化

Change of LPO,SOD and Ach in rat cortex during reperfusion following brain ischemia

本文采用四血管结扎法观察了大鼠全脑性缺血后血流再通三个时相脑皮质过氧化脂质(LPO)、超氧化物岐化酶(SOD)及乙酰胆碱(Ach)含量的变化。结果显示:血流再灌注期间脑皮质LPO明显增加,而SOD无明显变化,Ach的含量增加。说明脑缺血后血流再通所发生的再灌注损伤与氧自由基产生增加有关,而且可不伴有皮质对自由基清除系统功能的受损。同时说明再灌注损伤过程也牵涉皮质的胆碱能系统。

The transient brain ischemia was produced in rat with method of four vessels occclusion. Changes of lipid peroxidation (LPO). superoxide dismutase (SOD) and acetylcholine (Ach) in rat cortex were sbserved after 20 min. of brain ischerrda and during three phases of reperfusion. The results showed that in period of reperfusion, the LPO in cortex increased;and the Ach rose Up as well. However. there was not obvious change in the content of SOD. This results may indicate that the reperfusion injury following ischemia is closely related to the increase free radical generation which may not be due to the damage of the radical clearing system of brain. In addition, the reperfusion injury also involved to the cortical cholinergic system.