中国大陆水稻黄矮病与台湾省水稻暂黄病的血清学鉴定

Characterization of China's Mainland Rice Yellow StuntDisease and Taiwan Rice Transitory Yellowing Disease by Serological Identification

以前的文献报道我国大陆水稻黄矮病和台湾省的水稻暂黄病的病状、传毒介体和病毒形状均相似或相同,被认为是同一病害,但没有做过血清学反应的鉴定比较。本试验采用琼脂扩散法和双抗体夹心法(PAS-ELISA),对上述两病原的抗血清与黄矮病株提取液和带毒黑尾叶蝉研磨液进行了琼脂双扩散和酶联免疫反应的比较研究。黄矮病毒提取液与暂黄病毒抗血清的琼脂双扩散产生清晰的沉淀带,在ELISA试验中均为典型的阳性反应;黄矮病毒抗血清和暂黄病毒抗血清对生物测定虫的同一头黑尾叶蝉研磨液的测定结果,阳性虫附合率98％,病原田捕捉虫的符合率为100％。根据以上结果可以认为两种抗血清同源,即中国大陆水稻黄矮病与台湾省水稻暂黄病为同一病害。

The symptoms, virion morphologies and insect vectors of rice yellow stunt disease occurred in China's Mainland and riee transitory yellowing disease found in Taiwan are very similar or identical as deseribed in the previous reports, therefore they are putatively regarded as the same. But serological comparison between the causative viruses of two diseases has not been done so far. In this paper we report on the reactions of crude Rice Yellow Stunt Virus (RYSV) preparations extracted from diseased rice plants and Viruliferous leafhoppers to antisera against to RYSV and Rice Transitory Yellowing Virus(RTYV)with agarose-gel immunodiffusion test and Protein A indirect sandwich ELISA (PAS-ELISA). The extracts containing RYSV and the antiserum to RTYV produce clear sediment bands in agarose-geldiffusion tests and show typical positive reactions in ELISA tests. The positive reaction coincidence rates of two antisera to halves of the same leaf hoppers are 98% to 100％, According to the above results, we conclude that two antisera are homologous and further confirm that rice yellow stund disease and rice transitory yellowing disease are essentially the same.