摘要
本文描述了从自然罹死的斜纹刺蛾(Oxyplax orhracea(moore))幼虫中分离出一种核型多角体病毒。用快速简便方法提取核酸,经限制性内切酶EcoRⅠ、HindⅢ酶解,获得该病毒核酸的酶解带谱。以λDNA的EcoRⅠ酸解片段在凝胶中的迁移率与相应DNA片段分子量的对数值做标准曲线求得其平均分子量为102.09×10~6道尔顿,即为147.77kb。
The morphology of Oxyplax ochracea (Moore) Nuclear Polyhedrosis Virus (OoNPV) isolated from naturally infected larvae is described in this paper. The endonuclease digestion map of OoNPV-DNA is obtained by restriction endonucleases EcoRI and HindⅢ digesting, The average molecular weight of OoNPV is 102.09×19~6d, i.e. 147.77kb.
出处
《中国病毒学》
CSCD
1993年第2期158-163,共6页
Virologica Sinica
关键词
斜纹刺蛾
多角体病毒
限制性内切酶
Oxyplaz ochracea Nuclear Polyhedrosis Virus Morphology Restriction endonucleases map
