应用原位杂交及免疫组化检测肝活检组织中流行性出血热病毒RNA及抗原

Studies on Liver Biopsy of Epidemic Hemorrhagic Fever by in Situ Hybridization and Immunohistochemistry

本文应用原位杂交及免疫组化技术对25例流行性出血热(EHF)患者肝活检组织进行了病毒RNA及其囊膜G_2蛋白的定位检测,结合光镜,认为肝细胞的变性、胞浆疏松化、点状坏死是EHF病毒直接侵犯并在其胞浆内增殖表达所致,肝组织的灶状坏死则主要是肝窦狭窄、枯否氏细胞增生导致微循环障碍引起的缺血性梗死。急性脂褐素沉积是病毒侵犯肝细胞的间接证据。研究还发现,肝细胞内病毒的多少与病程关系不明确,而与临床分型有一定相关,为探讨EHF的发病机制提供了分子水平的依据。

The EHF RNA and its G_2 antigen were detected by in situ hybridization and immunohistoche- mistry on liver biopsy specimens of 25 cases of EHF patients among whom 23 are postive. Our results indicated that the degeneration and the single hepatocyte necrosis were due to the EHF virus invading hepatocytes directly and replicating in their cytoplasm; the local hepatocyte necrosis may be a kind of ischemia infarct which was mainly because of micro-circulation disturbance owing to the narrow of heaptocellular sinus and the hyperplasia of Kupffer's cell. Comparing the amount of vius RNA in hepa- tocyte between different clinical types of EHF, it showed that the positive signals in middle, heavy types were much stronger than that of in light type.