成人腹泻轮状病毒蛋白的免疫印迹分析

Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus

我们从腹泻病人便样中纯化了病毒,其结构蛋白组分按分子量大小分别为VP1(136K),VP2(113K),VP3(92K),VP4(84K),VP5(64K),VP6(47K),VP7(41K)。所有这些蛋白皆具有抗原性。WP6是B组轮状病毒的共同抗原。B组轮状病毒的每一结构蛋白与A组轮状病毒都无交叉免疫反应。另外注意到二例不同病人对VP6和VP7刺激产生的抗体水平不同。

Adult diarrhea rotavirus (ADRV) is known as only one of group B rotavirus that causes human diarrhea. The viral proteins were determined by SDS-PAGE of purified virions from infected feces as follows: VP1 (136K), VP2 (113K), VP3 (92K), VP4 (84K), VP5 (64K), VP6 (47K), VP7(41K). Western blot analysis was used to examine their immunological properties. The results showed that all structural polypeptides are antigenic, VP6 is most strongly one and contains a common group B anti- gen. The rtatio of the antibody response to VP7 and VP6 differed markedly between two patients. No cross-reactivity could be demonstrated with antisera to group A rotavirus tested by western blot of ADRV polypeptides or antisera to ADRV tested against group A proteins.