摘要
本文在Rat-2(TK^-)细胞上,对浣熊痘病毒TK基因作为病毒筛选标记的作用进行了研究。发现浣熊痘病毒虽然具有TK基因的序列和功能,但对5-溴脱氧尿嘧啶(5-BUdR)药物的选择压力并不敏感。即使采用HAT培养基挑选的单斑病毒对5-BUdR也具有抵抗力。
In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. Southern blot showes that RCN has TK gene sequence though it hybridizes week ly with vaccinia virus TK gene. But RCN is resistant to 5-BUdR even after it was plaque purified in HAT media.
出处
《中国病毒学》
CSCD
1993年第4期338-340,共3页
Virologica Sinica
