Behcet综合征患者淋巴细胞和单核细胞中C-myc和ras癌蛋白的表达及NK亚群状况的研究

NK subsets and the expression of C-myc and ras oncoproteins in lymphocytes and monocytes from patients with Behcet's syndrome

作者在21名Behcet综合征(Behcet's syndrome,BS)患者和40名健康人外周血淋巴细胞和单核细胞中检测了C-myc、K-ras和H-ras三种癌蛋白的表达及天然杀伤细胞(natural killer,NK)亚群的状况。结果发现,BS组NK中CD16^+,57^-亚群细胞数显著低于对照组,患者淋巴细胞中三种癌蛋白的表达均显著地高于对照组;单核细胞中C-myc和K-ras蛋白的阳性率也明显高于对照。相关分析表明,BS组淋巴细胞中C-myc的表达与血清γ-球蛋白的百分比,IgM、IgG水平呈正相关关系,患者单核细胞中C-myc的表达与血沉速率呈正相关关系,与NK的CD16~ +,57^-亚群细胞数呈负相关关系;淋巴与单核细胞中K-ras和H-ras的表达均与血清IgA水平呈负相关关系。作者对上述实验结果的意义和BS与自身免疫之间的可能关系进行了分析讨论,认为自身免疫很可能是导致BS的重要机制。

The expression of C-myc, K-ras and H-ras oncoproteins in lymphocytesand monocytes, and NK subsets were studied in 21 patients with Behcet's syndrome (BS)and 40 healthy individuals. CD16^+,57^- cells of NK from BS patients were much decreasedwhen compared to the control. The positive percentages of three kinds of oncoprotein in BS lymphocytes and C-myc and K-ras oncoproteins in BS monocytes were significantly higher thanthat of the control. Positive correlations were found not only between C-myc expression oflymphocytes and serum γ-globulin%, IgM and IgG levels in BS, but also between C-mycexpression of monocytes and ESR. Expression of K-and H-ras oncoproteins in lympho-cytes and monocytes from BS patients were found to be negatively correlated with serumIgA level. Negative correlation was also found between C-myc expression in monocytesand CD16^+,57^- subset of NK. The significance of these results and the possible correlationsbetween BS and autoimmunity were discussed. It was suggeted that autoimmunity mightbe an important mechanism underlying BS.