摘要
本实验采用Langendorff灌流装置造成大鼠心肌缺血/再灌模型,用差速离心法分离线粒体,用Clark氧电极测定RCR、ADP/O,用铁氰化钾脉冲法测定了呼吸链电子传递及质子泵出初速度。结果表明,H^+/2e在缺血时与对照比无显著差异,而再灌组显著低于对照,H^+/2e下降早于ADP/O下降。RCR、ADP/O在缺血早期及再灌时显著高于对照。
Ischemia and reperfusion of rat hearts were produced by Langendorff per-fusion technique. The energy tranduction of the mitochondria isolated from the rat heartswas studied. The initial rate of proton ejection and eldectron transfering were measured.There was no significant difference of H^+/2_e ratio between the ischemic group and thecontrol group which was reduced only in the reperfusion group. The longer was the is-chemic period before reperfuaion, the smaller would be the H^+/2_e ratio. The lowering ofH^+/2_e ratio occurred earlier than that of the ADP/O ratio. RCR and ADP/O in shortperiod ischcmia group and reperfusion group were both much higher than that of the con-trol group.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1993年第5期561-564,共4页
Chinese Journal of Pathophysiology
关键词
缺血
再灌注
线粒体
心肌
能量代谢
Ischmia
Perfusion
Mitochondria
Heart
Energy metabolism
Oxidative phosphorylation
