血小板激活因子对内皮细胞形态参数影响的计算机定量分析

Computerized quantitative analysis of the effects of platelet-activating factor on endothelial cell morphological parameters

利用具有大容量、高运算速度和高分辨率的计算机图像分析系统建立定量分析内皮细胞(endothelial cell,EC)面积、形状因子、细胞间距及细胞间空隙面积占细胞单层面积百分比的软件,并分析血小板激活因子(platelet activating factor,PAF)对EC形态参数的影响。结果表明,对照组EC相互连接紧密,细胞间隙较窄。PAF作用60min,细胞明显回缩,膜表面有很多粗细不等的突起,细胞间隙增大。计算机定量分析表明,PAF(10^-?mol/L)作用10min,就可使细胞间距和细胞间空隙面积占细胞单层面积百分比增大,30min细胞面积减小,形状因子增大,证明细胞发生了回缩,这可能是PAF增加血管壁通透性的重要机制。计算机图像分析有助于迅速、准确地定量测定EC形态变化,为研究EC在血管屏障中的作用提供了新方法。

Software for quantitative analysis of endothelial cell (EC) area, form fac-tor, intercell-plasma-membrane distance and percentage of intercellular gap area in the cellmonolayer was established in a computer image analysing system with great capacity, highspeed and high resolution. The effects of platelet activating factor (PAF) on EC morpho-logical parameters were studied. The results showed that the untreated EC monolayer hadclose contact and narrow gap among cells. After 60 min treatment with 10^(-8)mol/L PAF,the cells showed obvious retraction, with procession of different size from the plasma mem-brane and increased intercellular gap. Computerized quantitative analysis revealed thatthe treatment with PAF for 10 min increased intercellular distance and percentage of inter-cellular gap area in cell monolayer. Thirty minutes treatment decreased cell area butincreased form factor ascertaining to the retraction of EC. This is probably an importantmechanism of increased vascular permeability induced by PAF It is also suggested thatcomputerized image analysis is helpful in rapid, precise and quantitative measurement ofEC morphological changes. It provides a new method for studying the effects of endothe-lial cells in increased vascular permeability.