ELISA检测低硒小鼠组织GSH—Px的含量——兼论GSH—Px含量与活性的关系

An assay for GSH—Px protein content in various tissues of selenium deficient mouse by ELISA-A discussion on the correlation between the GSH—Px activity and its protein content

昆明小鼠30只分别饲以低硒(14.7ng/g)及补硒(500ng/g)等饲料27天,用 ELISA 检测心、肝、肾、肝细胞线粒及胞质 GSH—px 含量,结果表明肝>肾>心,低硒动物组织及肝亚细胞成分酶含量明显下降,补硒则可维持酶含量和活性,本方法灵敏性高,方法简便。低硒时动物组织内 GSH—px 含量下降幅度不及全血酶活性大,揭示低硒早期,动物组织内可能存在无活性蛋白多肽,作者对其发生机制进行了探讨。

The Kunming mice were fed with Se-deficient(14.7ng/g Se)or a Se-supplemented(500ng/g Se)as Na_2SeO_3 composed diet for 27 days.The changes of GSH—Px protein level in heart,liver,kidney,mito-chondria and cytosol of liver cell were examined by Sandwich enzyme linked immunosorbent assayingmethod(ELISA).The results indicated that the liver had the highest level of GSH—Px protein,then was thekidney and heart.All the tissues and subcellular portions of the liver cells of Se-deficient mice showed signifi-cent decrease in GSH—Px protein,and the Se-supplemented diet had very significent influence on maintain-ing the normal GSH—Px activity and its protein content.Our experiments also indicated that the ELISA wasa very simple and sensitive method for examing the GSH—Px protein.The correlations between the GSH—Px activity and its protein content in Se-deficient mice were discussed.