硒锌对培养软骨细胞生长代谢的影响

Effects of selenium and zinc on the growth metabolism of cultured chondrocytes

本文报告了硒、锌对培养软骨细胞生长代谢的影响。本实验在培养液中加入一定量硒、锌对一代人胚关节软骨细胞进行培养192h。结果显示:(1)补锌和补硒均能促进软骨细胞的生长。(2)同时补充适量的硒和锌比单独补充效果好,但不能超过正常人血清含量的中等水平,若过多补充则可能适得其反。(3)在同一阶段,软骨细胞的分裂增殖与其产生基质的量不一致。(4)体外培养软骨细胞的基质代谢能很快进入平衡状态。胶原较蛋白多糖的代谢为慢。

In this pater,the effects of selenium andzinc on the growth metabolism of culturedchondrocytes were reported.Human embryoarticular chondrocytes had been recultured inthe media supplied with selenium and zinc for129 hours.The result showed:(1)zinc and se-leneum supplied in the media at a given con-centration could all promot the growthmetabolism of cultured chondrocyte;(2)theeffects were better when both zinc and seleni-um were supplied at the same time.But theconcentration can not be over the mediumlever of normol human serum.The concentra-tion over the normel level lead to the oppositeresult;(3)in the same period,the reproductionof chondrocytes and matrix were not equal inquantity;(4)the matrix metabolism of cul-tured chondrocytes could get balanced quick-ly.The metabolism of the collagen was slowerthan the proteoglycan.