不育(孕)症精液及子宫颈粘液抗精子抗体检测

Detection of anti-spermatozoa antibodies in the semen or the cervical mucus among the inferitility

目的 :了解抗精子抗体 (AsAb)及其类型与不育 (孕 )症的关系。方法 :采用ELISA法检测AsAb ,支原体采用培养法 ,衣原体用金标法。结果 :12 0例不育症精液和 36例不孕症子宫颈粘液AsAb总阳性率为 2 6 .2 8% ;其中精液阳性率为 2 0 .83% ,子宫颈粘液阳性率为 44 .40 % ,男女间差异有显著性 (P <0 .0 1)。抗体类型中 ,IgM型男性阳性率 2 .5 0 % ,女性为 8.33 % (P>0 .0 5 ) ;IgG型男性阳性率 6 .6 7% ,女性为 36 .11% (P <0 .0 0 5 ) ;IgA型男性阳性率 12 .5 0 % ,女性为 30 .5 6 % (P <0 .0 5 )。精液中IgM和IgA间差异有显著性 (P <0 .0 1) ,但IgG与IgA和IgM间差异均无显著性 (P >0 .0 5 ) ;子宫颈粘液中IgM分别与IgA和IgG间差异均有显著性 (P <0 .0 5和P <0 .0 1) ,但IgG与IgA间差异无显著性 (P >0 .0 5 )。同时进行支原体、衣原体检测 ,感染与否和AsAb(+ )间差异均无显著性 (P >0 .0 5 )。结论 :精液和子宫颈粘液AsAb是不育 (孕 )症的重要原因 ,以IgG和IgA型为主 ;

Objective:To discuss the relationship of sperm antibodies and the antibody types with inferitility.Methods:The anti sperm atozoa antibodies(AsAb) were examined by ELISA,the infection of ureaplasma urealyticum(UU) and mycoplasma hominis(MH) by culture and chlamydia trachomatis(CT) by dot immuno gold fitration assay(DIGFA).Results:The total AsAb positive rates in the 120 semen and the 36 cervical mucus were 26.28%,among them,the positive rates in semen were 20.83% and the AsAb(+) rates in the cervical mucus were 44.40%.There was significant difference between male and female patients( P <0.01).In the antibody types,the types of IgM's positive rates were 2.50% in the semen and 8.33% in the cervical mucus( P >0.05);the IgG rates were 6.67% in the semen and 36.11% in the cervical mucus( P <0.005);the IgA rates were 12.50% in the semen and 30.56% in the cervical mucus( P <0.05).The difference between IgM and IgA in the semen was significant( P <0.01),but there was no great difference among IgG,IgA or IgM( P >0.05).The difference among IgM,IgA or IgG in the cervical mucus was significant( P <0.05 and P < 0.01 ),but the difference between IgG and IgA was not( P >0.05).There was no significant difference between infection and noninfection in AsAb(+)( P >0.05).Conclusions:The results indicate that AsAb(+) plays an important role in sterility especially the IgG and IgA types.The infection of MH,CT and UU has no influence on producing AsAb(+).