VIP对大鼠离体逼尿肌肌条自发性收缩作用的实验研究

Experimental study of VIP on automatic contractility of isolated detrusor strip

目的 观察VIP对离体逼尿肌肌条自发性收缩频率及收缩幅度的作用。方法 建立Wistar大鼠下尿路梗阻动物模型 ,6周后行充盈性膀胱测压 ,根据逼尿肌稳定性将下尿路梗阻动物分为逼尿肌不稳定组和逼尿肌稳定组 ,用拉力传感器观察血管活性肠肽 (vasoactiveintestinalpeptide,VIP)对离体逼尿肌肌条的自发性收缩频率及收缩幅度的影响。 结果 大鼠膀胱下尿路梗阻六周后逼尿肌不稳定发生率为 62 .1 % ;VIP能明显抑制逼尿肌的自发性收缩 ,降低逼尿肌肌条自发性收缩频率及收缩幅度 (P <0 .0 5) ;梗阻后逼尿肌不稳定组、逼尿肌稳定组及对照组之间无显著差异 (P >0 .0 5)。结论 VIP能显著抑制正常和梗阻后逼尿肌自发性收缩 ,但其在逼尿肌不稳定情况下的作用仍需深入研究。

Objective To investigate the effects of vasoactive intestinal peptide (VIP) on obstructive of the range and the frequency of the automatic contractility on isolated detrusor strip.Methods On the basis of making the model of obstructive of Wistar rats low urinary tract(LUT),the cystometry was detected after 6 weeks, the animals were divided into two groups with or without detrusor instability(DI):DI and non-DI group,then observe the effects of VIP on the range and the frequency of the automatic contractility on isolated detrusor strip.Results The rate of DI after 6 weeks′ obstructive of LUT was 62.1%; VIP could inhibit the contract of detrusor significantly, decrease the range and the frequency of the automatic contractility (P< 0.05 ),with no differences on the DI, non-DI and the control group(P>0.05).Conclusion VIP can inhibit the contract of normal and post-obstructive detrusor significantly,but their action among the DI still need to have a further study.