王不留行及其伪品的蛋白质电泳鉴别

Electrophoresis identification of semen vaccariae and its adulterants

目的 对王不留行及伪品进行了电泳鉴别 ,并考察考马斯亮蓝G - 2 5 0的染色效果及电泳方法的效果。方法 可溶性蛋白质凝胶电泳。结果 王不留行及伪品的电泳图谱存在显著差异。结论 电泳图谱可作为王不留行及伪品的鉴别依据 ,所用电泳方法简便、快捷 ,考马斯亮蓝G - 2 5 0可用于中药的电泳鉴别。

Objective To identify semen vaccariae and its adulterants by electrophoresis and research the dyeing effect of Coomassie Brilliant Blue G-250.Method Gel electrophoresis of soluble protein was used.Results There was a significant difference between the electrophoretograms of semen vaccariae and its adulterants.Conclusion The electrophoretograms can be used to differentiate semen vaccariae and its adulterants.Coomassie Brilliant Blue G-250 can be used in the electrophoresis identification.