平滑肌细胞源性趋化因子的研究

A Study On Smooth Muscle Cell—derived Chemotactic Factor

以兔主动脉平滑肌细胞条件培养基作为趋化因子的来源,用微孔滤膜法检测其对平滑肌细胞及NIH 3T3纤维母细胞的趋化活性。结果表明,该条件培养基对培养的平滑肌细胞有明显的趋化活性,而对NIH 3T3纤维母细胞则否;该条件培养基对平滑肌细胞的迁移作用是一种趋化作用而非化学促动作用。

The medium conditioned by cultured rabbit aortic smooth muscle cells (SMC—CM) was used as the source of chemotacic factor. Its chemotactic activity for smooth muscle cells (SMCs) and NIH 3T3 fibroblasts were examined by mieroporous filter assay. The results showedthat the SMC—CM was chemotactic for SMCs but not for NIH3T3 fibroblasts,and the effects of SMC—CM on SMC migration were considered to be chemotacic rather than chemokinetic in nature.