平滑肌细胞源性生长因子对NIH 3T3纤维母细胞DNA合成的影响

Effects of Smooth Muscle Cell—derived Growth Factors on the DNA Synthesis of NIH 3T3 Fibroblasts

动脉中膜平滑肌细胞(SMCs)迁移进入内膜和增生是动脉粥样硬化发生的关键环节,并受包括PDGF、IGF、FGF、EGF在内的多种因素的影响。众所周知,SMCs自身亦可合成及分泌生长因子。未研究以平滑肌细胞条件培养基及平滑肌细胞粉碎物作为生长因子的来源,观察其对~3H—TdR掺入NIH3T3细胞DNA的影响,结果提示两者均促进NIH 3T3细胞DNA的合成,说明平滑肌细胞合成的生长因子部分被分泌到细胞外并可与其自身细胞膜上的生长因子受体结合,部分保留在细胞内促进其自身的增生。

Migration of smooth muscle cells (SMCs) into subendothelial space and their proliferation were key events in the atherogenesis, and were influenced by a variety of factors including PDGF, FGF, IGF, EGF,etc. It was known that SMCs could synthesize and secrete growth factors in a paracrine and autocrine manner. The SMCs conditioned medium (SMCs—CM) and the SMCs—sonicate (SMCs—son) were used as the source of growth factors,and their influence on the DNA synthesis of NIH 3T3 fibroblasts by ~3H—Thymidine incorporation. The resuits showed that SMCs—CM and SMCs—son both promoted the DNA synthesis of NIH 3T3 fibroblasts. These date suggest that growth factors synthesized by SMCs could be partly secreted out cells,among which some could bind with the receptors on SMC memberane,and the rest remained within the cells,stimulating the cells' prolifeartion.