脑梗死患者血管紧张素原基因M235T分子变异的相关性研究

The Correlation Between Variant of the Angiotensinogen gene M235T with Cerebral Infarction

目的 探讨血管紧张素原(angiotensinogen AGT)基因M 235 T分子变异与中国人脑梗死(cerebral infarction,CI)之间的关系。方法 采用聚合酶链反应(PCR)及限制性片段长度多态性分析(RFLP)法对75例CI、48例健康对照进行了AGT基因M 235 T多态性检测。结果 CI组AGT基因T 235等位基因频率为78.0％,235 TT基因型频率为64.0％。与对照组(分别为60.4％、37.5％)比较差异具有显著性(χ2=8.82,P=0.003;χ2=8.27,P=0.004)。校正了CI的几种危险因素(血总胆固醇、血糖及年龄)后,235 TT基因型仍可使CI发生的危险性增加(分别为OR=3.289,P=0.036;OR=2.49,P=0.023)。结论 AGT基因235 TT型可能是中国人群CI发病的独立危险因素。

Objective To investigate the correlation between angiotensinogen (AGT) gene M235 T variant and cerebral infarction (CI) in a Chinese population. Methods AGT gene M 235 T polymorphism was examined in 75 cases with CI and 48 healthy persons by polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP). Results The frequencies of AGT gene 235 TT genotype were 78.0% and 64.0% respectively. Compared with those of the control group (60.4% and 37. 5% respectively), the difference was significant ( x2 =8.82, P=0.003; x2 =8.27, P = 0.004). After three CI risk factors (glycemia, cholesterol and age) had been adjusted, 235 TT genotype could still raise the incidence of CI (OR = 3.289, P =0.036; OR =2.49, P =0.023). Conclusions 235 TT genotype of the AGT gene might be an independent risk factor of CI in a Chinese population.