高血压病人红细胞ATPase,细胞离子和游离钙的变化

The Changes of Erythrocyte ATPase,Intracellular Ions and Free Calcium in Patients with Essential Hypertension

本研究对 39例轻、中度高血压病 (EH)患者 ,通过皂素溶血定磷法测定红细胞ATPase活力 ,原子吸收火焰法测定红细胞离子含量 ,Fura 2 /AM荧光探针测定红细胞 [Ca2 +]i含量 ,并以正常人作对照 ,旨在探讨部分EH的发病机制。结果显示 ,EH组红细胞Ca2 +ATPase活力明显降低 ,P <0 .0 1,而Na+ K+ATPase无显著性差异 ,两者之间呈正相关 (r =0 .46 ) ;红细胞Na+和 [Ca2 +] i含量 ,均显著增高 (P <0 .0 1和 0 .0 0 1) ,而细胞K+含量明显降低 ,以致Na+/K+比值增大 (均P <0 .0 1)。研究表明 ,EH患者红细胞高Na+,高 [Ca2 +] i及低K+,是膜缺陷及阳离子转运异常的标志或结果 ,是部分EH患者发病的病理基础和分子生物学基础。红细胞Na+泵活力正常 ,Ca2 +泵活力抑制 。

The erythrocyte Ca 2+ ATPase,Na + K +ATPase and Mg 2+ ATPase as well as intracellular Na +,K +,Ca 2+ ,Mg 2+ and free calcium ([Ca 2+ ]i) contents were investigated in patients with essential hypertension(EH).The method of saponin to hemolysed RBC was used to determine the activity of erythrocyte ATPase;flame method to determine intracellular ion content and fluorescent indicators of Fura 2/AM loaded RBC to determine intracellular [Ca 2+ ]i content in 39 mild to moderate EH subjects and healthy volunteers.The results showed that in EH group the activity of erythrocyte Ca 2+ ATPase was inhibited (P< 0.01 ),and the activity of Na + K +ATPase was normal.There was a good correlation between Ca 2+ ATPase and Na + K +ATPase (r=0.46).The intracellular Na + and [Ca 2+ ] i contents were markedly elevated (P<0.01 and 0.001),whereas intracellular K + content was markedly lowered.These results indicate that the intracellular elevated Na +,[Ca 2+ ]i and lowered K + in EH subjects were responsible for abnormal membrane transport of cation and abnormalization of plasma membrane.These changes may be regarded as the essential pathogenesis of some patients with hypertension.The normal activity of erythrocyte Na + K +ATPase and the inhibited Ca 2+ ATPase may be regarded as a clinical type of EH.