幽门螺杆菌vdcA基因多态性与慢性胃病的关系

Relation between diversity of vacA of Helicobacter pylori and chronicgastric diseases

目的探讨幽门螺杆菌(HP)vdcA基因多态性与慢性胃病的关系。方法经胃镜检查活检胃粘膜标本,分离鉴定Hp,酚氯仿方法抽提DNA,聚合酶链反应检测vdcA基因的信号区(s)和中间区(m)。结果92例Hp临床分离株中,sla/ml型14株(15.2)、sla/mla型6株(6.5％)、s2/m2型8株(8.7％)、slb/ml型2株(2.2％)、slb/m2型5株(5.4％)、s2/m2型8株(8.7％),另在2株slaHP不能按ml、mla或m2进一步分型,未发现s2/ml、slb/mla及s2/mla型。除4例胃癌外,其余88例按消化性溃疡和慢性胃炎分组,40例消化性溃疡患者感染Hp中sla型37例,占92.5％,slb型2株(5％),s2型1株(2.5％);ml型4株(10％),mla型2株(5％),m2型32株(80％),另有2株sla型Hp未能按ml、mla或m2进一步分型。48例慢性胃炎患者感染Hp中sla型36株,占75％,slb型5株(10.4％),s2型7株(14.6％):ml型11株(22.9％),mla型4株(8.3％)m2型33株(68.8％)。消化性溃疡组和慢性胃炎组均以sla型、m2型为主,分别比较两组间sla型及m2型的分布,两组间sla型有显著性差异(P<0.05),而m2型无显著性差异(P>0.05)。结论慢性胃病患者感染Hp主要为sla/m2型,其次为sla/ml型。Sla型Hp与消化性溃疡的发生密切相关。

AIM To analysis relation between the diversity of Helicobacter pylori and chronic gastric diseases. METHODS Gastric biopsy specimens for culture were taken by using a sterilized endoscope, 92 strains H. pylori clinical isolates from patients were cultured and collected.DNA was extracted by the phenol-chloroform method, vacA signal sequences and middle-region alleles were amplified by the method of polymerase chain reaction(PCR) RESULTS 92 clinical isolates of Hpylori were characterized by vacA typing,14(15.2％)of 92 isolates wcre sla/m 1.6(6.5％)were sla/mla, 55(59.8％)were sla.m2.2(2.2％)were slb/ml.5(5.4％)were slb/m2.8(8.7％)were s2/m2.only 2 could not betyped s2/ml or slb/mla or s2/mla isolates had not been found.37(92.5％)of 40 isolates from patients with peptic ulceration were sla,2(5％)were slb,1(5％)were slb,1(2.5％)was s2;4(10％)were m1.2(5％)were mla,32(80％)were m2,the midregions from2isolates from patients with peptic ulceration could not be typed 36(75％)of 48 isolates from patients with chronic gastritis were sla,5(10.4％)were slb,7(14.6％)were s2;11(22.9％)were ml.4(8.3％)were mla,33(68.8％)wwere m2 sla and m2 isolates were found more frequently.The form sla between two groups was statistically significant (P<0.05),but the form m2 between two groups was not different(P>0.05). CONCLUSION These results indicate that the sla/m2-type strains aer common in the patients from south-china The sla forms correlate with peptic ulceration.