海尔曼螺杆菌感染小鼠的病理学研究

Pathological investigation of mouse model of Helicobacter hellmanli infection

目的建立海尔曼螺杆菌(Hh)感染小鼠模型,以了解 Hh 感染小鼠的病理学特点。方法50只Ⅱ级 Balb/c 小鼠随机分为实验组(30只),实验组小鼠采用由 Hh 感染患者转归的小鼠胃粘膜组织磨碎后直接灌喂方法建立 Hh 感染小鼠模型,而对照组不作相应处理。于灌喂后第1、2、4、8、及16周分批处死小鼠,每批处死实验组小鼠6只、对照组小鼠4只。取胃粘膜组织分别进行细菌培养、涂片Gram 染色、快速尿素酶试验(RUT)、组织切片 Warthin-Starry 银染、组织病理学检查。结果 Hh 容易在 Balb/c 小鼠胃内定植,成功率达到100%。在感染第4周始,部分 Hh 感染小鼠胃粘膜可见少量淋巴细胞、中性粒细胞和嗜酸性粒细胞浸润,至感染第16周,全部感染小鼠胃粘膜均可见不同程度的淋巴细胞、浆细胞浸润,部分小鼠伴有中性粒细胞漫润,而对照组小鼠未见有明显的炎细胞浸润。结论 Hh 可长期稳定定植于小鼠胃内,并可引起慢性活动性胃炎,可用于 Hh 的致病性研究。

Objective To establish mouse model infected with Helicobacter heilmanii(Hh)and investigate the pathological characteristics of Hh infection. MEthods 50 two-grade Balb/c mice were randomly divided into tow groups, experimental and control group. The mouse gastric mucosa tissue infected with Hh from a patient ground and inoculated oraly to 30 experimental mice to establish mouse model. In 1, 2, 4, 8, 16 weeks after the bacteria inoculation, 6 experimental mice and 4control mice were sacrificed respectively. Histology and Hh colonization were assessed by cultuer, smears Gram stainig, rapid urease test, Warthin-Starry silver staining, and histological examination. Results Hh was prone to colonize in gastric mucosa of all experimental Balb/c mice After 4weeks of infection. there were a few lymphocytes, nerutrophilic leukocytes, acidophilic leukocyes in gastric mucosa of some infected mice. After 16 weeds of infection.much more lymphocytes and plasmocytes could be seen in all the infected mice to some degree, and a few neutrophilic leukocytes could be seen in all the infeced mice to some degrees, and a few neutrophilic leukocytes in gastric mucosa occurred in some infectd mice. In contrast, n sigificalnt cell infiltration was seen in control mice. Conclusion Hh can colonize steadily in gasric mucosa of Balb/c mice and induce chronic active gastritis in long-term study, which can be used to researc pathogenicty of Hh.