血管内皮生长因子表达与大肠癌血管生成、转移的关系

目的检测血管内皮生长因子(VEGF)、血小板反应素(TSP)在大肠癌中的蛋白表达,及其与大肠癌微血管计数、转移之间的关系。方法:采用免疫组化方法(S-P法)检测105例原发性大肠癌手术切除组织中的微血管计数(MVC)和VEGF、TSP蛋白表达。结果:105例大肠癌中VEGF表达阳性率为61.9％,而TSP表达阳性率为(72.4％)。 MVC在VEGF表达阳性者明显高于VEGF表达阴性者(31.6±13.2vs25.4±10.7,P<0.05)。VEGF表达阳性率在发生淋巴结转移、远处转移病人明显高于无转移者(P均<0.05)。MVC在TSP表达阳性者和阴性者之间没有显著性差异(28.7±12.8vs30.8±12.4,P=0.434)。TSP表达与淋巴结转移和远处转移无关。结论:VEGF表达与大肠癌血管生成和转移密切相关,可作为反映大肠癌发展和转移潜能的生物学指标。

Objective The aim of this study was to analyze the relationship of vascular endothelial growth factor, thrombospondin expression with angiogenesis and metastasis in colorectal adenocarcinoma. Method The microvessel counts (MVC), the expression of VEGF, TSP protein in 105 primary colorectal carcinoma specimens were evaluated by immunohistochemical staining.Results:Sixty-five (61.9%) of the 105 cases were positive for VEGF protein, seventy-six (72.4%) positive for TSP protein. MVC was significantly higher in VEGF-positive tumors than in VEGF-negative tumors (31.6±13.2 vs 25.4±10.7. P<0.05). VEGF expression was more frequent in lymph node(P<0.05) and hematogenous(P<0.05) metastatic tumors than in non-metastatic tumors. MVC was not different between TSP-positive tumors and TSP-negative tumors(28.7±12.8 vs 30.8±12.4, P=0.434). TSP expression showed no association with lymph node and hematogenous metastasis.Conclusions:The expression of VEGF is closely associated with angiogenesis and tumor metastasis, it could be regarded as a biological marker in prognosis for colorectal tumor progression and metastasis.