矽肺患者血清N-乙酰-β-D氨基葡萄糖苷酶的活性及其临床价值研究

Serum N-acety1-β-D-glucosaminidase Activity in Silicosis Patients and Its Clinical Value

本文利用分光光度法测定了50例矽肺患者、18例0~ +期接尘工人、29例0期接尘工人及51例正常对照受检者血清N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性。结果表明,正常对照受检者血清NAG活性(X±SD)为10.27±1.64u/L,矽肺患者为16.94±4.52u/L,0^+期接尘工人为14.63u/L。矽肺患者与0^+期接尘工人血清NAG的活性经统计学检验无显著性差异,但二者均显著高于0期接尘组(9.56±2.6u/L)和正常对照组。本实验测得皿清铜蓝蛋白的结果表明,矽肺患者血清铜蓝蛋白高于正常对照组和0期接尘工人,但0^+期接尘工人与0期接尘工人无显著性差异。矽肺患者血清NAG与铜蓝蛋白之间存在正相关性。

Serum NAG was measured using a color-metric method with the substrate of PNP-NAG when serum ceruloplasmin was assayedusing the photoelectrometric method of Bauer.The subjects of this study included 50 silicosispatients,18 0^+ cases, 29 silica-exposed work-ers and 51 normal subjects. The result showedthat serum NAG level (mean±SD) in normalsubjects was 10.27±1.64u/L, and it slightlyincrease with age. Serum NAG levels in silicoticand of cases were 16.94±4.52u/L and 14.63±4.59u/L, respectively. The difference betweenthe both groups was not statistically sign-ificant. Serum NAG levels in silicotic and 0^+cases were significantly higher than that ofsilica-exposed workers (9.59±2.gu/L) andnormal subjects. The results also showed thatserum ceruloplasmin levels in silicotic patie-nts were higher than those of normal andsilica-exposed workers. But the differencebetween 0^+ cases and silica-exposed workerswas not statistically significant.