两种化学物经皮肤渗透的离体动式模型与活体实验的比较

Flow-through Diffusion Cell as an in Vitro Model to Predict Percutaneous Absorption of Chemicals In Viro

本实验选用氚水及^(14)C-杀虫脒作为测试物,研究皮肤渗透的离体动式模型与活体实验的关系。经化学物皮肤吸收动态分析及动力学参数比较,均发现离体与活体实验结果相关。利用经皮肤渗透的离体动式模型进行实验研究,能估测化学物经活体皮肤吸收的速度和程度。

Based on the study of percutaneous absorption of chemi- cals in vitro with the static diffusion cell, a novel flow- through diffusion cell has been established in our laboratory. Compared with the static diffussion cell, it has the advantages of flowing receptive fluid and steady temperature, which are much closer to those conditions in vivo. and automatic sam- pling. Tritiated water and ^(14)C-labeled chlordimeform are stud- ied here. It is found that penetration rate and degree of tritiat- ed water and chlordimeform in vitro are similar to those of in vivo at the doses of 7.3×10~4Bq per cm^2 of triated water and 4. 4×10~6Bq per cm^2 of chlordimeform, respectively.