华支睾吸虫病肝脏病理组织学和组织化学的实验观察

HISTOLOGICAL AND HISTOCHEMICAL OBSERVATIONS ON THE LIVER OF GUINEA-PIGS WITH CLONORCHIS SINENSIS

动态观察实验性感染华支睾吸虫豚鼠肝脏病理变化的结果表明,光镜下,感染后2周始,汇管部胆管上皮脱落,渐呈乳头状增生,管壁周围纤维组织增殖且不断向小叶内伸展和大量炎性细胞浸润。肝细胞呈水样变性和局灶性坏死。7周后,胆管上皮腺瘤样组织和小胆管增生明显,胆管多显著扩张。肝细胞索解离,有空泡变性和溶解坏死。透射电镜下发现,感染早期,肝细胞核和一些膜性细胞器出现轻度病变,6周后,粗面内质网扩张成池,线粒体基质透明、空泡化。组织化学观察结果,示肝细胞内糖原、蛋白质、RNA减少或消失,胞质内出现脂肪滴,SDH、MAO活性呈渐进性减少。7周后,不断增殖的胆管和增生的小胆管处,糖原、RNA、粘蛋白含量明显增加,AKP、ACP有处呈强阳性。

Systemic and dynamic observations were made on pathological changes in the liv- er of guinea-pigs with Clonorchis sinensis . Under LM, 2 weeks after infcetion, desquamation of bile duct epithelial cells and papilliform proliferation in the lumen of the bile ducts were seen. Bile duct walls were infiltrated by a lot of inflammatory cells and circled by proliferative connective- tissues, which stretched to hepatic lobules progressively. There were hydropic degenerations and focal necroses of hepatocytes. 7 weeks late, adenomatoid tissue formation of bile duct epithelium and ductule proliferation were shown markedly. Severe dilation of the bile ducts, vacuolar degen- eration and lytic necrosis of hepatocytes and seperation of hepatic cords were also seen. Under TEM, 1 weeks after infection, some moderate pathological changes of the nucleus and organelles of the hepatocytes were found. From 6 weeks after infection, rER enlarged into pool, Mi col- lapsed and finally disappeared and bile salt deposited in cytoplasm. The results of histochemical examinations were as following. 2 weeks after infection, glycogen, protein and RNA decreased and then disappeared. Fat drops apppeared in the cytoplasm. Activity of SDH and MAO reduced gradually. From 7 weeks after infection, in the regions of the proliferative bile ducts and duc- tules, the amount of glycogen, RNA and mucin enhanced obviously, AKP and ACP presented more active.