308nm紫外激光对兔眼角膜的光化学损伤反应的研究

Study of ultraviolet-induced photochemical damage in rabbits' corneal irradiated with 308 nm XeCl excimer laser

近来,紫外激光和近紫外激光对生物组织的损伤作用研究引起世界众多学者的关注。本实验采用308nm XeCl准分子激光器作为照射源,以免眼角膜为动物模型,研究角膜受308nm紫外激光照射瞬时反应的损伤阈,并对角膜损伤延迟反应作一探讨,为制订我国激光防护标准提供依据。

Investigation of Chinchilla rabbits' corneal losions induced by 308 nm excimer laser was presented. The damage mechanism was the same as photo-chemical and there were two phases of reaction of immediate damage and delayed ones. By statistical analysis, the immediately corneal injury threshold (ED50) of 0. 485 J/cm2was obtained and its 90% confidence limit was 0. 349-0. 608 J/cm2. The delayed reaction appeared 6-18. hours after the irradiation.