激光消融对角膜细胞的影响

Corneal cell reactions to laser ablation

本文用计算机图像定量分析308nm准分子,193nm准分子和2.94μm Er∶YAG激光分别照射角膜后,角膜上皮细胞和内皮细胞的形态变化。结果表明:308nm激光对角膜上皮细胞有明显损伤;193nm对再生的上皮细胞无明显影响,而2.94μm对上皮细胞无影响。但若使用2.94μm激光能量密度过高或切削过深,则对内皮细胞有影响。

Different wavelength lasers (308 nm, 193 nm and 2. 94μm) were used to irradiate cornea. Morphological changes of epithelial and endothelial cells were observed by quantitative image analysis. It was found that epithelial cells of cornea irradiated by 308 nm excimer laser have apparent changes in nuclear area and morphology. The epithelial cells regenerated after 193 nm laser irradiation have no obvious change. After 2. 94μm laser irradiation, no change in nuclear morphology and area was observed. But it was harmful to endothelial cells, if energy density or ablating depth was excessive.