巨噬细胞呈递日本血吸虫抗原的作用

ROLE OF MACROPHAGE IN PRESENTING ANTIGENS OF SCHISTOSOMA JAPONICUM

以日本血吸虫卵尿素溶性抗原(UEA)经巨噬细胞(Mφ)加工后免疫同源小鼠,自免疫鼠体抗体水平变化探讨加工后的产物是否仍具有免疫原性及 UEA信息经 Mφ呈递给抗原识别细胞的方式。同时对比其与多聚甲醛固定的Mφ(P-Mφ)或氯化铵处理的Mφ(NH_4Cl-Mφ)分别被UEA或是胰酶消化的UEA(T-UEA)激活后免疫小鼠的抗体水平差异。以间接ELISA检测抗体水平。结果显示:Mφ被UEA激活后,以其细胞及培养上清免疫小鼠均可使抗UEA抗体水平明显升高,提示UEA经Mφ加工后仍具有免疫原性。抗原信息可由Mφ和上清传递;P-Mφ和NH_4Cl-Mφ与UEA作用后,免疫鼠血清的抗体水平升高,且前者高于后者,说明氯化铵对Mφ呈递抗原的影响较大;T-UEA激活的两种预处理的Mφ免疫鼠血清抗UEA抗体水平较用UEA激活的低,提示胰酶可能破坏Mφ表面抗原偶合结构或破坏UEA的免疫原性。

Indirect ELISA was employed to monitor the serum anti-UEA(urea soluble egg antigen of Schistosoma japonicum )antibody level of mice immunized by a. UEA pulsed macrophage (M + ); b. Cultural supernatant of M+; c. paraformaldehyde fixed M(P -M)pulsed with UEA; d. Ammonium chloride treated M0 (NH4Cl-M0) pulsed with UEA, e. P -M0 pulsed with trypsin digested UEA (T-UEA ); f. NH4C1-M pulsed with T-UEA. The normal M its supernatant and the culture media RPMI 1640 acted as the negative control.The results showed : 1. Serum anti-UEA antibody levels of mice immunized by a and b raised markedly, indicating that the immunogenicity of UEA might be kept up after M processing and the antigenic message could be transferred either by the M+ or by its supernatant; 2. Mice immunized by c and d gave similar results, but the anti-UEA antibody level of the former was higher than that of the latter, suggesting that polyformaldehyde could not alter the UEA binding site on the surface of M; 3. In the case of mice immunized by e and f , the antibody levels were much lower than that of mice immunized by c and d, suggesting that UEA binding sites on M surface as well as UEA immunogenicity could be changed by trypsin.