淡色库蚊越冬过程中蛋白质/多肽二维电泳分析

ANALYSIS ON THE PROTEIN/POL YPEPTIDE IN CULEX PIPIENS PALIENS (DIPTERA:CULICIDAE) DURING HIBERNATION BY TWO-DIMENSIONAL GEL ELECTROPHORESIS

用二维电泳对淡色库蚊越冬过程中蛋白质/多肽的变化进行了研究。分别查出未越冬蚊、越冬蚊及越冬复苏蚊分子量及等电点不同的蛋白质/多肽点153个、118个和169个。分子量在43kDa以下的斑点占79.08-89.83％,等电点在5.65-7.34的斑点占66.01-83.90％。越冬蚊与越冬复苏蚊的蛋白质/多肽点总差异率(31.01％),显著高于未越冬蚊与越冬复苏蚊的总差异率(21.12％)(P<0.01)。未越冬蚊、越冬蚊与越冬复苏蚊三者共有的蛋白质/多肽点为71个,特有的斑点分别为13个、7个和27个。越冬复苏蚊的蛋白质/多肽点的变异率(15.98％)显著高于未越冬蚊(8.50％)(P<0.05)及越冬蚊(5.93％)(P<0.01)。整个越冬过程中蛋白质/多肽点的总变异率为10.68％。因用于制备匀浆的蚊虫胃血均为Sellal期,卵巢发育不超过体止期,故可排除胃血及卵巢后期发育对结果的影响。

Analysis on the protein / polypeptide in Culex pipiens pallens ( Diptera : Culicidae) caught from the resting or overwintering places during the 3rd ten-day of September,the 2nd ten-day of December and the 1st ten-day of March in next year in Zhengzhou(34°43'N ,113° 39' E) during hibernation by two-dimensional gel electrophoresis indicated that there were 153, 118 and 169 protein/polypeptide spots in unoverwintering, overwintering and recovering mosquitoes respectively. The molecular weight (MW) of most of the spots was less than 43. 0 kDa, with isoelectric points (pI)between 5. 65-7. 34. The comparisons between each two types of mosquitoes during hibernation revealed that the variant rate of protein / polypeptide spots between overwintering and recovering mosquitoes (31. 01 % ) was significantly higher than that between unoverwintering and recovering females (21.12%) (P<0. 05). The variant spots were 13 in unoverwintering, 7 in overwintering and 27 in recovering mosquitoes respectively. The comparisons among three types of mosquitoes during hibernation showed that the variant rate was significantly higher in recovering females(15. 98%) than in unoverwintering (8. 50%)(P<0. 05)and overwintering females(5. 93%)(P<0. 01). The total variant rate of protein/polypeptide spots of all these three types of mosquitoes was 10. 68%.Because the blood meal of all the mosquitoes used for homogenization was at Sella 1 (empty), and the ovary development was under Christophers Ⅱ(resting stage), the experimental results were not affected by blood meal and the later ovary development (Christophers Ⅱ-Ⅴ).