感染疟原虫小鼠红细胞膜的流动性

FLUIDITY OF RED BLOOD CELL MEMBRANE FROM MOUSE INFECTED WITH MALARIA PARASITE

以DPH作为荧光探针,用荧光偏振度及微粘度作为指标,对感染伯氏疟原虫小鼠的红细胞膜及感染约氏疟原虫小鼠的红细胞膜的流动性进行了检测。结果显示,感染疟原虫小鼠的红细胞膜的荧光偏振度及微粘度均降低。与正常小鼠相比较均存在显著性差异,表明感染小鼠红细胞膜的流动性增高。本文尚对膜的流动性增高的机制进行了简要讨论。

The fluidity of membrane lipid regions of Plasmodium berghei- or Plasmodium yoelii- infected red blood cells has been determined by the fluorescence polarization technique using 1, 6-diphenyl-1, 3, 5-hexatriene (DPH) as a probe. The results showed that the fluidity of Plasmodium(berghei or yoelii)-infected red blood cell membranes was increased significantly as compared with that of normal controls judging from the degree of polarization and the microviscosity. Its mechanism was discussed briefly.