摘要
用DNA探针pHD5、pEG18、pSM889 对我国细粒棘球蚴与多房棘球蚴通过限制性内切酶 BamHI与 EcoRI 酶解后用 Southern 转移杂交法进行了虫种鉴别。该方法敏感、特异,尤其对在光镜下无法区分的标本更具有鉴别的优越性。它能够用于包虫病流行病学基线调查及监测,为包虫病的防治提供准确的依据。
Cloned DNA fragments pHD5,pSM889 and pEG18 have been used as DNA probes in the restriction endonuclease analysis and southern blot hybridization to characterize E. granulo-sus and E. multilocularis protoscolices from China. Southern blot hybridization method is sensitive, specific and has the advantage in identification over microscopic examination. The authors deem that it can be used in the base-line epidemiological survey and surveillance of hy-datid disease to provide data for hydatid disease control.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第3期198-200,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金 编号38970678
关键词
包虫病
虫科
鉴别
DNA探针
Echinococcus granulosus,Echinococcus multilocularis,hydatid disease,species characterization,DNA probe.
