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单克隆抗独特型抗体NP30用于日本血吸虫病血清学诊断的研究 被引量:2

A STUDY ON THE MONOCLONAL ANTI-IDIOTYPIC ANTIBODY NP30 FOR THE DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA
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摘要 本研究用单克隆抗独特型抗体NP30与日本血吸虫肠相关抗原(GAA)和可溶性虫卵抗原(SEA)检测了702份不同病期及正常人群中的血清抗体,结果显示,在急性感染时,NP30抗体的检出率为98%,与GAA(94%)和SEA(98%)的无差别。在慢性感染时NP30抗体的检出率为87%,与GAA(86%)的无差别,但低于SEA(98%)的。在正常人群中,上述3种的抗体假阳性率均为3%左右,无差别。NP30的抗体滴度几何均数在急性血吸虫感染时高于GAA的,低于SEA的,在慢性感染时低于后两者,提示NP30的抗体在血吸虫感染期间出现比较早,消退较快。上述结果提示,NP30可以替代虫源性抗原,用于日本血吸虫病诊断。 In this study the ability of the monoclonal anti-idiotypic antibody NP30 was tested as a substitute of diagnostic antigen in detecting antibody of Schistosoma japonicum from human sera by use of ELISA. The results showed that the seropositive rate was 98% with NP30 in the group of acute infection, which was comparable to 94% with gut associated antigens (GAA)and 98% with the soluble egg antigens (SEA); 87% with NP30 in the group of chronic infection which was comparable to 86% with GAA but lower than that of 98% with SEA. The false positive rate was about 3% for all three diagnostic antigens. The results also showed that the geometric mean titer (GMT) of antibody to NP30 was higher than that to GAA but lower than that to SEA in the acute infection group and the GMT of antibody to NP30 was lower than both those to GAA and to SEA in the chronic infection group,suggesting that the antibody to NP30 appeared earlier and decayed more quickly during the process of infection. The authors suggested that NP30 could be used for the diagnosis of schistosomiasis japonica.
出处 《中国寄生虫学与寄生虫病杂志》 CSCD 北大核心 1993年第3期195-197,共3页 Chinese Journal of Parasitology and Parasitic Diseases
基金 江苏省卫生厅委托项目
关键词 血吸虫 血清抗体 单克隆抗体 诊断 Schistosoma japonicum monoclonal antibody anti-idiotypic antibody serum antibody
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参考文献4

  • 1管晓虹,中国寄生虫学与寄生虫病杂志,1991年,9卷,261页
  • 2杨树勤,中国医学百科全书.医学统计学,1985年
  • 3管晓虹,上海免疫学杂志,1982年,2卷,5期,1页
  • 4管晓虹,中华医学杂志,86页

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