摘要
将日本血吸虫超声干卵混悬于6％琼脂液内作石蜡切片,贴于 PVC薄膜凹孔进行免疫酶染色试验(薄膜干卵切片 IEST),并与薄膜肝卵切片 IEST对比实验,检测 159份血吸虫病人血清,阳性率分别为 98.11％及 97.48％,两者间无显著性差异(P>0.05)。检测 63份健康人血清,假阳性分别为 2例及 1例。提示两法的敏感性和特异性相近。但两法检测阳性血清的反应强度(染色程度“+”值)经秩和检验比较结果,超声干卵切片反应平均强度(X±SD 为2.51±0.09)明显高于肝卵切片(1.81±0.08,P<0.05),超声干卵切片含虫卵切面多,虫卵较密集,虫卵成熟度较一致,均有助于试验结果观察,PVC 薄膜价廉,便于携带,值得推广应用。
Immunoenzymatic staining technique (TEST) using PVC-membrane coated with Schisto-soma japonicurn ultrasonicated dry egg(UDE) and liver egg PVC-IEST was carried out to detect specific antibodies in sera from 159 cases with schistosomiasis japonica, the positive rates being 98. l%(156/159)and 97. 5% (155/159) .respectively. The sera from 63 healthy individuals showed false positive reaction in 2 cases and 1 case,respectively,suggesting that the sensitivity and specificity of the two tests were similar. However,the positive intensity was higher in UDE section than in liver egg section(F>0. 05). As the UDE section could be stained more obvious and the PVC membranes are cheap and could be easily brought so that this method might be aplicable for practical purposes.
出处
《中国寄生虫学与寄生虫病杂志》
CSCD
北大核心
1993年第4期270-272,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
免疫酶技术
血吸虫病
日本血吸虫
Immunoenzymatic staining
Schistosoma japonicum
serology
PVC membrane
