阿仑膦酸盐诱导破骨细胞凋亡FAS基因的表达

The expression of FAS gene in apoptotic osteoclasts induced by alendronate

本研究采用１０－８Ｍ１，２５（ＯＨ）２Ｄ３诱导ＳＤ鼠骨髓细胞形成破骨细胞，通过检测细胞上ＦＡＳ基因的表达，探讨骨吸收因子阿仑膦酸盐的作用机制。骨髓细胞培养６天即有多核巨细胞形成，加入阿仑膦酸盐致终浓度为１００μＭ，继续培养４８小时后，发现用药组破骨样细胞及圆形单核细胞的前体细胞呈现凋亡的形态学特征：胞浆收缩，核固缩等。ＦＡＳ抗原是与细胞凋亡密切相关的细胞表面蛋白，用抗ＦＡＳ抗体免疫组化方法，检测凋亡的破骨细胞及其前体细胞上ＦＡＳ基因的表达，结果呈阳性，而未凋亡细胞呈阴性，提示阿仑膦酸盐导致的破骨细胞及其前体细胞的凋亡。

In order to investigate the mechanisms by which bisphosphonate(alendronate)inhibit bone resorption,brought about by osteoclasts.We have developed a new in vitro model to study osteoclast survival.1,25(OH) 2D 3 causes formation of multinucleated cells with several osteoclast characteristics in cultures of SD rat marrow.Osteoclast like cells were grown from SD rat bone marrow examined morphologically for features of apoptosis after treatment with alendronate for 48h.The definitive of apoptosis features are cytoplasmin contraction,chromatin condensation and nuclear fragmentation. The FAS antigens are protein on the surface of lymphocyte or other cells and relate with apoptosis.By immunohistochemistry method,we use anti FAS antibodies to determine whether apoptotic osteoclast and its progenitor cells express FAS gene.The result is that apoptotic cells were positive,and nonapoptotic cells were negtive.So it is proposed that FAS gene may be involved in the event of apoptosis of osteoclast and its progenitor cells treated by alendronate.