口腔肿瘤细胞核仁组成区定量与分型研究

A qualitative and typing study of the nucleolar organizer region in maxillofacial tumors

目的应用核仁组成区嗜银蛋白染色技术（AgNOR）辅助口腔肿瘤细胞学诊断，以提高确诊率。方法对有病理组织学结果对照的口腔颌面部肿瘤细胞学标本486例进行了AgNORs实验研究。结果显示口腔良性肿瘤细胞的AgNOR平均颗粒为1．72±0.39，恶性肿瘤细胞为4．64±1．75，两者呈显著性差异（P＜O.001）；各类型肿瘤细胞显示具有颗粒数量、形态与分布的不同。结论Ag－NOR实验对肿瘤细胞的良恶性判断具有较大价值，尤以某些口腔癌中，尽管其肿瘤细胞形态呈良性征表现，但若AgNOR有颗粒数的明显增多或形态上明显变异，仍可确立为恶性肿瘤诊断。

0bective Complementary cytodiagnosis of oral neoplasms applying AgNOR techniqiue to stain the nucleolar organizer region in order to prmote the rate of accurate diagnosis. Methods Argyophilic technique (AgNOR) was applied on 486 specimens of oral and maxillofacial tumor (383 benign and 103 malignant) confirmed histopathologically. Results The aver-age number of AgNOR granules in benign oral tumor cells was 1. 72 ±O. 39 and that in malignant tumor cells 4. 64 ±1. 75, showing sighficant difference (P<0. 001); various types of tumor cell showed difference in number, mor-phology and distribution of their granules. Conlusions It is suggested that AgNOR technique is of value in differential diagnosis of tumor cells,espeially in carcinomas of oral and drillofacial region. Although the morphology of tumor cells appear benign, it can still be diagnosed malignant if there is marked increase in number or changes in shape.