肿瘤细胞淋巴细胞混合培养诱导产生胶质瘤特异性CTL的实验研究

Experimental study of glioma-specific cytotoxic T-lymphocyte lines induced by cocultivation of lymphocytes and tumor cells

目的 通过自身肿瘤激活诱导产生高活性的胶质瘤特异性细胞毒T淋巴细胞（G－S－ChL）。方法应用淋巴细胞、肿瘤细胞混合培养（MLTC）方法，诱导产生G－S－CTL，采用3H－TdR释放法及免疫荧光法检测G－S－CTL的细胞毒活性及免疫表型。结果G—S－CTL与自身LAK相比，抗肿瘤活性大为提高，CD3＋、CD4＋、CD8＋和CD25＋细胞明显增多。结论G－S－CTL抗肿瘤活性及免疫表型类似于胶质瘤浸润的淋巴细胞（GIL），对脑胶质瘤过继免疫治疗具有一定的实用价值。

Objective To induce highly active glioma \ specific cy-totoxic T - lymphocyte (G - S - CTL) lines by stimulation of autologous tu-mor cells. Methods G- S - CTL were established by cocultivation of lym-phocytes and autologous tumor cells. The cytotoxic activity and phenotypes of G- S - CTL were detected by 3H - TdR - release assay and immunofluo-rescence. Results Compared with auto - LAK, those G - S - CTL showed much higher antitumor activity; CD3＋ 、CD4＋、CD8 ＋ and CD25 ＋ cells in-creased evidently. Conclusions These results suggested that the antitumor activity and immunophenotype of G - S－CTL are similar to those of glioma infiltrating lymphocytes (GIL) and have practical value in adoptive im-munotherapy of patiens with brain gliomas.