抗副溶血弧菌鞭毛血清的初步研究

Rapid serological identification of Vibrio parahaemolyticus by anti - H coagglutination

目的：研制抗副溶弧菌鞭毛血清。方法：选择TSA培养基。提取鞭毛抗原，将鞭毛抗原（0．1-0．6mg/ml）对豚鼠进行免疫，获得抗鞭毛血清。结果：血清效价测定，抗HL1为1：3200，抗HIZ为：1：25600，抗HL3为1∶25600。与20种其他细菌均无交叉反应。血清与副溶血弧菌凝集反应，阳性率95％。结论：TSA培养基是较为适宜诱导鞭毛抗原产生的培养基。血清特异性高，有较高的敏感性。抗副溶血弧菌鞭毛血清可用子对副溶血弧菌进行鉴别。

Objective: To produce the anti - H of V. parahaemolyticus serum. Methods: Select the medium TSA, abstract the H antigen, and the gUinea pigs were immunized. Results: The titer of anti -- HI.1 is 1∶ 3200, anti - HL2 is 1: 25600, anti - HL3 is 1: 25600. There were no cross - test to other 20 kinds of bacterium. The agglutinative test of serum were 95 % positive with V. P. isolate. Conclusion: the TSA is thesuitable medium to produce the flagella antigen of V. P. The serological specificity and sensitivity is satisfying.The anti - H coagglutination test is a rapid, specific, and inexpensive procedure for identifying V. P beyondprimary isolation.