雌激素缺乏脊柱神经肽P物质变化的实验研究

Neuropeptide substance P immunoreactive innervation of lumbar vertebrae in ovariectomized rats

目的阐明雌激素缺乏时椎体、椎间盘及小关节等部位ＳＰ免疫反应阳性神经纤维的变化规律。方法选用健康２月龄雌性ＳＤ大鼠９６只，随机分为卵巢切除（Ｏｖａｒｉｅｃｔｏｍｙ，ＯＶＸ）和假手术（Ｓｈａｍ－ｏｐｅｒａｔｅｄ，Ｓｈａｍ－Ｏ）两组。ＯＶＸ组行双侧卵巢切除术，Ｓｈａｍ－Ｏ组行假手术。分别于术后１５天、１、２、３、６、９月时每组各处死８只动物，切取Ｌ３～５脊柱小关节、棘上韧带、椎体和椎间盘。免疫组织化学Ｓ－Ｐ法观察各部位ＳＰ免疫反应阳性神经纤维分布。结果①ＯＶＸ后骨小梁及小梁膜上ＳＰ免疫反应阳性神经纤维增多，增粗。②软骨组织内ＳＰ反应阳性纤维增多；２月时椎间盘ＳＰ阳性反应物增加，分布至纤维环中带，３月时观察到纤维环内带，６月在纤维环髓核中观察到ＳＰ免疫反应阳性神经纤维。结论雌激素缺乏时大鼠腰椎椎体、椎间盘、小关节ＳＰ阳性反应神经纤维增多，与骨质疏松性腰背疼痛有密切关系。

Objective\ To study the alternative regulation of peptide substance P(SP)innervation in the body,intervertebral disc,and facet joint of lumbar vertebrae in estrogen deficient condition. Methods 96 2 month old female rats were randomly divided into two groups.The animals were bilaterally ovariectomized (48)and sham operated (48);of them for each time were sacrificed and their L3 5 vertebrae were collected at the end of 15 th day,1,2,3,6 and 9 th months postoperation.Vertebrae,discs,facet joints and supraspinous ligaments were observed by immunohistochemical S P anlaysis. Results \ SP immunoreactive positive nerve fibers increased in trabecular bone and cartilage of facet joint post OVX.SP positive nerve fibers were found in inner third of the annulus fibrosus and the nucleus pulposus in the sixth and ninth months. Conclusion\ SP immunoreactive positive nerve fibers increase in lumbar vertebrae, intervertebral disc,cartilage of facet joint under estrogen deficient condition,indicating that there is obvious relationships between SP and lumbago in pathogenesis of osteoporotic low back pain.