摘要
目的探讨骨疏康冲剂与钙剂联合应用防治维甲酸所致大鼠骨质疏松的疗效。方法选用３～４月龄Ｗｉｓｔａｒ大鼠６０只，随机分为正常对照组、模型组、骨疏康组、钙组和骨疏康加钙组。除正常对照组外，给其他各组灌服维甲酸（７０ｍｇ·ｋｇ－１·ｄ－１）２周，同时用药各组分别灌服骨疏康冲剂（８ｇ·ｋｇ－１·ｄ－１）、高效钙（７５ｍｇ·ｋｇ－１·ｄ－１）、骨疏康冲剂（８ｇ·ｋｇ－１·ｄ－１）加高效钙（７５ｍｇ·ｋｇ－１·ｄ－１）。实验第４周末处死大鼠，测定股骨骨密度；取胫骨上段制作脱钙切片，进行骨组织形态观察；取股骨行三点弯曲试验，测骨生物力学性能指标。结果模型组与正常组比较，股骨骨密度降低（Ｐ＜０．０５），胫骨上段骨组织形态发生典型骨质疏松改变，股骨生物力学性能降低（Ｐ＜０．０５）。骨疏康加钙组与模型组比较，股骨骨密度增高（Ｐ＜０．０５），胫骨上段骨组织病理变化明显改善，股骨生物力学性能增强（Ｐ＜０．０５）。结论骨疏康冲剂与钙剂联合应用有防治骨质疏松症的作用，能提高骨的抗变形、抗破坏能力，从而降低骨折发生的危险性。
Objective To study the therapeutic results of Gushukang(GK) combined with calcium in preventing and treating osteoporosis induced by retinoic acid in rats.Method 60 Wistar rats aged 3 4 months were randomly divided into five groups:control group,model group,GK group, Ca group and GK+Ca group.Except the control group,the other groups were given retinoic acid 70mg·kg -1 ·d -1 by gastrogavage for 2 weeks.Meanwhile,the GK group was fed GK 8g·kg -1 ·d -1 ,the Ca group calcium 75mg·kg -1 ·d -1 ,and the GK+Ca group GK 8g·kg -1 ·d -1 and calcium 75mg·kg -1 ·d -1 for 4 weeks.At the end of the 4th week,all rats were sacrificed.The femur bone mineral density (BMD) was measured.Three point bending test was carried out for the measurement of the femur biomechanical properties.Proximal tibiae were decalcified for histomorphological observation.Result Compared with the control group,the femur BMD and biomechanical properties in the model group markedly decreased ( P <0 05);the tissue microstructure of the proximal tibiae showed obvious pathological changes of osteoporosis.In the GK+Ca group,compared with the model group,the femur BMD and biomechanical properties markedly increased ( P <0 05);the pathological changes of the proximal tibiae tissue microstructure significantly improved.Conclusion GK combined with calcium is effective in preventing and treating osteoporosis,improving bone biomechanical properties and reducing the risk of fracture.
出处
《中国骨质疏松杂志》
CAS
CSCD
1999年第2期74-77,共4页
Chinese Journal of Osteoporosis
基金
"九五"国家科技攻关计划资助
