耐药伤寒流行菌株的耐药谱及质粒图谱分析

PLASMID PROFILE ANALYSIS AND ANTIBIOTICS SUSCEPTIBILITY TESTING OF MULTIRESISTANT S. TYPHI

1989～1990年从四川达县地区分离的63株流行性伤寒沙门氏菌中,55株(87.3％)为多重耐药菌,仅对头孢呋肟、丁胺卡那霉素和新型氟喹诺酮类抗菌药物敏感。多重耐药的类型以SMKMABPCCPTCSUGMCET和SMKMABPCCPTCSUCET为主。同期收集的散发性伤寒沙门氏菌43株,有9株(21％)为多重耐药菌。质粒图谱显示,多重耐药菌都有1条分子量约为98Md的大质粒。其中1株还带有 3条质粒带。质粒消除试验证明,98Md的大质粒与多重耐药性有关。

From 1989 to 1990, 63 epidemic strains of S. typhi were collected in Daxiang area. 55 strains(87.3%) of them were multiresistant to either 7 or 8 antibiotics, and susceptible to cefuroxime, amikacin and new fluoroquinolones. In the same period, 43 sporadic strains of S. typhi were collected in our hospital. 9 strains(21%) were multiresistant. There were susceptive to most antibiotics except streptomycin, tetracycline and SMZ/TMP. Most of multiresistance patterns were SMKMABPCCPTCSUG MCET and SMKMABPCCPTCSUCET. Agarose gel electrophoresis of pla-smid DNA showed that all multiresistant strains had a large plasmic band with a molecular weight of about 98 millidalton (MD). After elimination of plasmid it was proved that 98 MD plasmid was responsible for the multiresistance of the typhoid bacilli.