摘要
借反相高压液相色谱法分离了钒(Ⅴ)和镍(Ⅱ)的5-Br-PADAP螯合物、于Zorbax CN柱上,用含10^(-2)mol/L KHSO_4-NaAc缓冲剂(pH 3.5)的70%甲醇-30%水(V/V)作流动相(0.7ml/min)可在18分钟内将此二螯合物分离并于550nm处检测。检测限为0.3 ng V(V)和2 ng Ni(Ⅱ),此方法已应用于合金钢和钒渣中钒的测定,以及水样中钒和镍的测定。
The 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) chelates of vanadium (Ⅴ) and nickel (Ⅱ) have been separated by reversed-phase high-pressure liquid chromatography. The metal chelates, in 50% aqueous ethanol solution, are separated within 18 rain on a Zorbax CN column with 10^(-2) mol/L bisulphate-acetate buffer (pH 3.5) in methanol-water(70: 30V/V) as mobile phase, at a flow rate of 0.7 ml/min, and then detected spectrophotometrically at 550 nm. The detection limits for V(Ⅴ) and Ni(Ⅱ)are 0.3 and 2 ng, respectively. The method has been applied to the determination of vanadium in alloy steel and of vanadium and nickel in water samples.
基金
The Project Supported by National Natural Science Foundation of China.
关键词
钒
镍
液相色谱
PADAP
测定
reversed-phase high-pressure liquid chromatography, vanadium, nickel, 2-(5-bromo-2-pyridylazo)-2-diethylaminophenol, alloy steel sample, water sample
