Fas和FasL在丙型肝炎肝组织中的表达及意义

The expression of Fas and FasL in liver tissue infected by hepatitis C virus and its significance

目的了解丙型肝炎患者肝细胞损伤与Fas抗原表达的关系。方法采用免疾组织化学方法显示Fas抗原与Fas配体（FasL）在丙型肝炎肝组织的分布，并同时运用原位杂交方法，检测肝组织中的丙型肝炎病毒（HCV）RNA。结果Fas抗原主要位于肝细胞胞浆。Fas抗原表达阳性细胞在肝小叶中多呈散在或灶状分布，汇管区周围和碎屑样坏死区内阳性表达较强。FasL大多位于肝内浸润的淋巴细胞，某些病例肝细胞胞浆也可呈弥漫强阳性。Fas抗原表达的阳性率与肝组织炎症程度相关。结论在丙型肝炎患者，Fas抗原表达水平与炎症活动度有关，未发现（HCV）RNA原位杂交阳性细胞与表达Fas抗原的肝细胞之间的对应关系。

Purpose To explore the relationship between liver cell damage and Fas antigen expression in liver of patients with hepatitis C. Methods Using the immunohistochemical staining to detect Fas antigen and Fas ligand in the liver. In situ hybridization was per formed to detect HCV RNA in the liver. Results Fas antigen was mainly in the cytoplasm of hepatocytes. These positive cells were scattered or clustered in the liver lobule. The posivtive signals were stronger in the periporta1 region and 'piecemeal necrosis' area. Fas iigand was located in the infiltraing lymphocytes,occasionally in the hepatocytes. The positivity of Fas antigen expression was correlat ed with the grade of liver inflammation activity. It was not found that the relationship between the positive cells of HCV RNA and Fas antigen. Conclusions In HCV infection,Fas expression in hepatocytes in up-regulated according with the sevrity of liver inflamma tion. The Fas system may play and important role in liver cell injury by HCV infection.