体外诱导大肠癌细胞分化及其碱性磷酸酶活性的变化

The chang of alkaline phosphatases activities in large intestinal cancer cells induced in vitro

目的观察单独和联合使用全反式维甲酸（ＡＴＲＡ）和１，２５－二羟维生素Ｄ３对大肠癌细胞株ＬｏＶｏ碱性磷酸酶（ＡＬＰ）活性的变化的影响。方法用不同剂量的ＡＴＲＡ和１，２５－二羟维生素Ｄ３单独或联合在体外作用于ＬｏＶｏ细胞２、４和６天，用脱氧胆酸钠溶解细胞，动力学方法测定ＡＬＰ活性，紫外吸收法测定蛋白质含量。结果单独或联合使用ＡＴＡＲ和１，２５－二羟维生素Ｄ３均可使ＬｏＶｏ细胞的肠型ＡＬＰ活性逐渐升高，而以联合用药效果更好（Ｐ＜０．０５）。结论联合使用Ａ－ＴＲＡ和１，２５－二羟维生素Ｄ３诱导ＬｏＶｏ细胞比单独用药效果更好。

Objective To observe the change of alkaline phosphatases (ALP) activities in large intestinal cancer cells line (LoVo) induced by all trans retinoic acid (ATRA) and/or 1,25-dihydroxy vitamin D 3。 Methods The large intestinal cancer cell line was treated by different dosages of ATRA and/or 1,25-dihydroxy vitamin D 3 for 2,4 or 6 days. Then, the cells were dissolved in sodium deoxycholate. The ALP activities were measured by dynamics assay and the total protein was detected by ultraviolet absorbency method. Results The intestinal type of ALP was gradually increased by drug treatment and the effects of combinative using two drugs were better. Conclusion The differential effects of combinative using ATRA and 1,25 dihydroxy vitamin D 3 on LoVo cells were better than that of using each drug only. It had clinical significance for the diagnosis and treatment on large intestinal cancer.