加味四逆散防治肝损伤的实验研究

Effects of jia We Si Ni San (JWSNS) on Liver Injury

本实验旨在观察加味四逆散抗急性肝损伤的作用。一是采用四氯化碳一次性皮下注射造成急性肝损伤大鼠模型，检测血清ALT、AST、SOD、MDA、GSH水平及肝组织MDA、GSH水平。二是采用D-氨基半乳糖造成急性大鼠肝损伤模型，检测血清SOD、MDA、GSH水平，并作病理学观察。三是观察加味四逆散对小鼠的急性毒性作用。结果表明：①加味四逆散能降低CCl4所致的急性肝损伤大鼠模型血清升高的ALT、AST及MDA值，并使降低的SOD及GSH值升高；②加味四逆散能降低CCl4所致的急性肝损伤大鼠模型肝组织中升高的MDA值，并使降低的GSH值升高；③加味四逆散能降低D－GalN所致的急性肝损伤大鼠模型血清中升高的MDA值，并使降低的SOD及G或值升高；④加味四逆散能使D－GlaN所致的急性肝损伤大鼠模型肝脏的病变程度明显减轻，尤以变性、坏死的程度减轻明显；⑤加味四逆散对小鼠的半数致死量LD50为305．80±32．55g／kg。研究表明，加味四逆散具有防治CCl4、D－GalN所致急性肝损伤作用，其抗肝损伤的作用机制与其降酶、抗过氧化作用有关，且该制剂口服安全无毒。

To investigate the protective effects of JWSNS on acute liver injury and the acute toxic effect. Two kinds of liver injury models wer employed by ip injection of Carler tetrachloride(CCl4) and D - galac tosamise (D - GalN) respectively . Serum alanine aminotransferase (ALT), asparae aminotransferase (AST) and superoxide dismutase (SOD), glutathion (GSH ) activites, malondialdehyde(MDA) conent in serum, GSH activity and MDA content in liver tissue were measared. The histopathological effects of JWSNS was observed. The results sugysted:①JWSNS could decrease ALT, AST activihes and MDA content, but improv SOD and GSH activities in acute liver injury induced by CCl4 -intoxication; ②JWSNS could decrease MDA content in liver tissue, but improve its GSH activity in acute liver injury induced by CCl4; ③JWSNS could decrease serum MDA content, but improve serum SOD and GSH activities in acute liver injury induced by D - GalN; ④JWSNS could lessen steatois degree, especially inhibit the hepatocytes degeneration and necrosis; ⑤The median lethal dose of JWSNS (LD50 ) was 305. 80±32. 55 g/kg. JWSNS had the marked effects of preventing liver injuly, which related to its effects of decreasing ALT, AST activities and anti - lipid peroxidation in liver injurp. And JWSNS was safe.