幽门螺杆菌感染所致胃粘膜上皮细胞凋亡与p53和bcl—2表达的关系

目的阐明幽门螺杆菌(Helicobacter pylori,Hp)感染对胃粘膜上皮细胞凋亡的作用及其与 p53和 bc(?)-2表达的关系。方法本研究观察了28例幽门螺杆菌 Hp 阳性和17例 Hp 阴性慢性浅表性胃炎患者和20例 Hp 阴性正常胃粘膜对照者胃粘膜上皮细胞凋亡百分率及其 p53和bc(?)-2表达水平。结果:正常胃粘膜和 Hp 阴性慢性浅表性胃炎患者胃粘膜上皮细胞凋亡百分率低,p53蛋白低表达和 bc(?)-2蛋白高表达。而 Hp阳性慢性浅表性胃炎患者胃粘膜上皮细胞凋亡百分率高,p53蛋白高表达而 bc(?)-2蛋白低表达。结论 Hp 可诱导胃粘膜上皮细胞凋亡,其作用途径与上调 p53蛋白表达水平及下调 bc(?)-2蛋白表达水平有关。

Aims:To clarify the effects of Helicobacter pylori(Hp)on gastric epithelial apoptotic rates and expression,oncogenes p53,bcl-2 of Materials and methods:Using flow cytometry technique,we observed the gastric epithelial apoptotic rates and oncogenes p53.bcl-2 expression in 28 cases of Hp positive gastri- tis,17 cases of Hp negative gastritis and 20 cases of normal gastric mucosa.Results:Low levels of gastric epithelial apoptotic rates and p53 expression and high levels of bcl-2 expression were showed in both nor- mal gastric mucosa and Hp negative gastritis patients.However,high levels of gastric epithelial apoptotic rates and p53 expression were showed,and low levels of bcl-2 expression were showed in Hp positive gas- tritis patients.Conclusion:Hp could induce gastric epithelial apoptosis,which is related to the upregula- tion of p53 and downregulation of bcl-2.