实验脾虚证胃肠粘膜细胞的增殖动力学研究

Study on Proliferative Kinetics of Gastrointestinal Mucosal Cells in Experimental Spleen Asthenia Syndrome

目的探索脾虚时胃肠粘膜细胞的增殖能力。方法应用抗-BrdU 的单克隆抗体和免疫组织化学技术分析胃肠道粘膜细胞的增殖情况。结果 BrdU 的免疫标记显示,脾虚时胃肠粘膜细胞的增殖能力下降(P<0.01),经四君子汤预防和治疗的脾虚大鼠胃肠粘膜细胞的增殖能力明显增强,与脾虚自然恢复大鼠有显著差异(P<0.05或 P<0.01)。结论脾虚时胃肠粘膜细胞的增殖能力低下,四君子汤能显著改革其增殖状态。

Aim:To explore proliferative activity of gastrointesitnal mucosal cells in spleen asthenia syndrome.Methods:Proliferation of gastrointestinal mucosal cells was analyzed with monoclonal antibody specific to BudU and immunohistochemical technique.Results:BrdU-immunohistochemical technique demonstrated that proliferation of gastrointestinal mucosal cells was prohibited in spleen asthenia syn- drome.Prolifferative activity of gastrointestinal mucosal cells could be increased and even better than spontaneous recovery group after prevention andtherapy with Si Jun Zi decoction(SJZD).Conclusions: The study confirmed that proliferation of gastrointestinal mucosal cells was prohibited in spleen asthenia syndrome,which implied that general regulation ofadaptability and metabolism was in disorder and was an objective evidence for exploring the essence of spleen asthenia syndrome.