摘要
目的研究一氧化氮(NO)在肝癌发生发展的作用。方法用免疫组化的方法对21例肝癌及癌旁组织中的3种一氧化氮合酶(NOS)及血管内皮细胞生长因子(VEGF)的表达进行原位检测和观察,结果紧邻癌细胞的肝硬化组织或慢性肝炎组织iNOS呈强阳性,远离癌组织的肝硬化组织或慢性肝炎组织多呈阴性或弥漫弱阳性;iNOS在周边癌组织及侵入纤维组织中的癌细胞呈阳性,癌组织核心多呈阴性或弥漫弱阳性。VEGF、nNOS的分布与iNOS相似。eNOS主要分布在肝癌细胞小血管壁内皮及其肌层组织。结论 NOS表达与肝组织癌变及肝癌侵润能力有关,与癌组织获得血管形成和转移表型有关。
To investigate the effect of nitric oxide on tumor progression of hepatocelulor carcinoma (HCC). Methods The epression and localization of vascular endothelial growth factor (VEGF)、 constructive and inducible nitric oxide synthase (cNOSiNOS) in the 21 patients with HCC were studeid by immunohistochemistry. Results The iNOS protein was markedly expressed at the iver tissue of hepatic cirrhosis or chronic liver hepatitis, which was close to tumoredge. Meanwhile it was weakly or undetetable at the liver tissue localized beyond 1.5 cm from the tumor edge. The iNOS protein was markedly expressed at the invasjve tumor edge and HCC cells that invasived into stroma, but it was weakly or undetectable at the most tumor core. The expression of VEGF and nNOS were similar to that of iNOS' s. The eNOS protein was mainly detected at the endothelium and smooth muscle cells of tumor vessels. Conclusion Excessive expression of NOS may contribute to the pathogenesis and invasion of HCC.
出处
《现代消化及介入诊疗》
1999年第2期29-31,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology